草业科学-叶绿素荧光成像系统Weed-PAM
基于功能强大的IMAG-PAM,改进升级后适用于野外杂草,草坪等光合作用研究
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主要功能
l 成像功能:对Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等至少17种参数进行成像分析。测定调节性能量耗散Y(NPQ),反映植物光保护能力,测定非调节性能量耗散Y(NO),反映植物光损伤程度。
l 程序测量功能:可程序测量荧光诱导曲线、快速光曲线和暗弛豫,也可手动测量;在测量过程中能自动分析所有荧光参数的变化趋势
l AOI功能:可在测量前或测量后任意选择感兴趣的区域(AOI),程序将自动对选择的AOI的数据进行变化趋势分析,并在报告文件中显示相关AOI的数据。所有报告文件中显示的数据都可导出到EXCEL文件中。
l 成像异质性分析功能:对任意参数任意时间的成像,可在图像上任意选取两点,软件自动对两点间的数据进行横向异质性分析,并可导出到EXCEL文件中。
l 成像数据范围分析功能:对任意参数任意时间的成像,可分析任意两个荧光数值之间有多少个像素点,多少面积(cm2)。
l 突变株筛选功能:可跟据成像结果快速筛选光合、产氢/油、抗逆(抗盐、抗旱、抗病等)等突变株。
l 吸光系数测量功能:快速测量叶片的吸光系数。吸光系数测量光源: 16个红光(650 nm)和16个近红外(780 nm)LED,用于测量植物叶片或藻类样品PAR吸光系数。
成像参数
l Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等
技术参数
Maxi探头(蓝光版)蓝色LED,450 nm
Maxi探头(红光版)红色LED,650 nm
Mini探头(蓝光版)蓝色LED,460 nm
Mini探头(红光版)红色LED,620 nm
应用领域
l 光合作用研究:可以在相同的条件下同时对大量样品进行成像
l 植物病理学:病斑部位(包括肉眼不可见时)成像以及病斑扩散的时空动力学
l 植物胁迫生 理学:肉眼不可见胁迫损伤的早期检测
l 遗传育种:出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株
l 突变株筛选:快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等
l 分子生物学:宏观水平上检测样品的绿色荧光蛋白(GFP)荧光
l 其它多种扩展研究
参考文献
数据来源:光合作用文献 Endnote 数据库,更新至 2021 年 1 月,文献数量超过 10000 篇
原始数据来源:Google Scholar
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